Recombinant Full Length Human PSMD4 Protein, His tagged

• Cat.No.: PSMD4-02HFL
• Product Overview: Recombinant protein of human proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (PSMD4), full length, with N-terminal HIS tag, expressed in E. coli.

Specification

• Description: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21.
• Source: E. coli
• Species: Human
• Tag: N-His
• Protein length: 1-377 aa
• Molecular Mass: 40.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Notes: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Storage Buffer: 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% sarkosyl, 10% glycerol
• Concentration: >0.05 μg/μL as determined by microplate BCA method

Gene Information

• Gene Name: PSMD4 proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 [ Homo sapiens ]
• Official Symbol: PSMD4
• Synonyms: PSMD4; proteasome (prosome, macropain) 26S subunit, non-ATPase, 4; 26S proteasome non-ATPase regulatory subunit 4; AF; AF 1; Rpn10; S5A; angiocidin; RPN10 homolog; antisecretory factor 1; S5a/antisecretory factor protein; multiubiquitin chain-binding protein; 26S proteasome regulatory subunit S5A; ASF; AF-1; MCB1; pUB-R5
• Gene ID: 5710
• mRNA Refseq: NM_002810
• Protein Refseq: NP_002801
• MIM: 601648
• UniProt ID: P55036