Recombinant Human Protein Tyrosine Phosphatase, Receptor Type, C, His-tagged

• Cat.No.: PTPRC-1622H
• Product Overview: Recombinant human PTPRC (604-end) was expressed in Sf9 insect cells using an N-terminal His tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: 604-end a.a.
• Description: PTPRC contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. PTPRC has been shown to be an essential regulator of T and B cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling.
• Applications: Phosphatase Assay; Western Blot
• Molecular Weight: 95 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM MOPS, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
• Specific Activity: 11500 nmol/min/mg
• Purity: > 90 %
• Concentration: 0.1 ug/ul
• Sequences: 604-end
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Adaptive Immunity Signaling; Axon guidance; B Cell Receptor Signaling Pathway; BCR signaling pathway; CXCR4-mediated signaling events; Cell adhesion molecules (CAMs); EPO Receptor Signaling; Fc gamma R-mediated phagocytosis; Immune System; Phosphorylation of CD3 and TCR zeta chains; Primary immunodeficiency; Sema4D in semaphorin signaling; Semaphorin interactions; T Cell Receptor Signaling Pathway; TCR signaling

Gene Information

• Gene Name: PTPRC protein tyrosine phosphatase, receptor type, C [ Homo sapiens ]
• Official Symbol: PTPRC
• Synonyms: PTPRC; protein tyrosine phosphatase, receptor type, C; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180; receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; OTTHUMP00000033813; OTTHUMP00000033816; OTTHUMP00000033817; OTTHUMP00000038574; leukocyte common antigen; leukocyte-common antigen; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide; EC 3.1.3.48; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen
• Gene ID: 5788
• mRNA Refseq: NM_002838
• Protein Refseq: NP_002829
• MIM: 151460
• UniProt ID: P08575
• Chromosome Location: 1q31-q32
• Function: hydrolase activity; protein binding; protein kinase binding; protein tyrosine phosphatase activity; receptor activity; transmembrane receptor protein tyrosine phosphatase activity