Active Recombinant Mouse PTPRC protein, hFc-tagged

• Cat.No.: PTPRC-2172M
• Product Overview: Recombinant Mouse PTPRC protein(NP_035340.3)(Met1-Leu429), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Leu429
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized human SEMA4D-His at 10 μg/ml (100 μl/well) can bind mouse PTPRC-Fc with a linear range of 0.625-5 μg/ml.
• Molecular Mass: The recombinant mouse PTPRC /Fc is a disulfide-linked homodimer. The reduced monomer comprises 643 amino acids and has a predicted molecular mass of 71.7 KDa. The apparent molecular mass of the protein is approximately 118 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ptprc protein tyrosine phosphatase, receptor type, C [ Mus musculus ]
• Official Symbol: PTPRC
• Synonyms: PTPRC; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; L-CA; lymphocyte antigen 5; leukocyte common antigen; lymphocyte common antigen; loc; B220; Cd45; Ly-5; T200; CD45R; Lyt-4
• Gene ID: 19264
• mRNA Refseq: NM_001111316
• Protein Refseq: NP_001104786