Recombinant Human Protein Kinase N3, GST-tagged

• Cat.No.: PKN3-1084H
• Product Overview: Recombinant full-length human PKN3/PRK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: PKN3 or PRK3 is a serine/threonine protein kinase which can associate with RHOA in a GTP-dependent manner. PKN3 can also interact with GRAF and GRAF2 via the SH3 domains and phosphorylate these proteins. PKN3 is a mediator of malignant growth of human prostate cancer cells and functions downstream of activated PI3K. PKN3 is upregulated in human tumor cells but not in surrounding nontumorigenic tissues. Inducible knockdown of PKN3 expression inhibited metastasis in a mouse prostate tumor model.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 130 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50mM Tris-HCL, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF,0 25% glycerol.
• Specific Activity: 3 nmol/min/mg
• Purity: > 75 %
• Concentration: 0.1 ug/ul
• Sequences: Full length
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Salmonella infection

Gene Information

• Gene Name: PKN3 protein kinase N3 [ Homo sapiens ]
• Official Symbol: PKN3
• Synonyms: PKN3; protein kinase N3; RP11-545E17.1; serine/threonine-protein kinase N3; OTTHUMP00000022293; protein kinase PKN-beta; protein-kinase C-related kinase 3
• Gene ID: 29941
• mRNA Refseq: NM_013355
• Protein Refseq: NP_037487
• MIM: 610714
• UniProt ID: Q6P5Z2
• Chromosome Location: 9q34.13
• Function: ATP binding; nucleotide binding; protein binding; protein kinase C activity; protein kinase activity