Active Recombinant Full Length Human PKN3 Protein, GST-tagged

• Cat.No.: PKN3-6880HF
• Product Overview: Full-length human PKN3[1-889(end) amino acids of accession number NP_037487.2] was co-expressed as N-terminal GST-fusion protein (127 kDa) with CDC37 and HSP90a using baculovirus expression system. GST-PKN3 was purified by using glutathione sepharose chromatography and gel filtration chromatography.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: GST
• Protein Length: 1-889
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Purity: 71%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws. Use within 12 months from the date of shipment.

Gene Information

• Gene Name: protein kinase N3
• Official Symbol: PKN3
• Synonyms: PKN3;UTDP4-1;PKNbeta
• Protein Refseq: NP_037487.2
• UniProt ID: Q6P5Z2