Recombinant Human Protein Kinase, CAMP-Dependent, Regulatory, Type I, Beta, His-tagged

• Cat.No.: PRKAR1B-4872H
• Product Overview: Recombinant full-length human PRKAR1B was expressed in Sf9 insect cells using a C-terminal His tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: PRKAR1B is the type I-beta regulatory subunit of cyclic AMP-dependent protein kinase A (PKA) which is an essential enzyme in the cAMP signaling pathway. PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP. PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. PKA undergoes a dramatic conformational change upon complex formation with the catalytic subunit.
• Applications: Western Blot
• Molecular Weight: 52 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
• Specific Activity: 871 nmol/min/mg
• Purity: > 90 %
• Concentration: 0.2 ug/ul
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Apoptosis; Aquaporin-mediated transport; Ca-dependent events; CaM pathway; Calmodulin induced events; DARPP-32 events; Diabetes pathways; EGFR1 Signaling Pathway; G Protein Signaling Pathways; G-protein mediated events; Hemostasis; Insulin signaling pathway; Integration of energy metabolism; Opioid Signalling; PKA activation; PKA activation in glucagon signalling; PLC beta mediated events

Gene Information

• Gene Name: PRKAR1B protein kinase, cAMP-dependent, regulatory, type I, beta [ Homo sapiens ]
• Official Symbol: PRKAR1B
• Synonyms: PRKAR1B; protein kinase, cAMP-dependent, regulatory, type I, beta; PRKAR1; cAMP-dependent protein kinase type I-beta regulatory subunit; OTTHUMP00000199947; OTTHUMP00000199948; OTTHUMP00000199949; OTTHUMP00000199952; NP_001158230.1; EC 2.7.11.1
• Gene ID: 5575
• mRNA Refseq: NM_001164758
• Protein Refseq: NP_001158230
• MIM: 176911
• UniProt ID: P31321
• Chromosome Location: 7pter-p22
• Function: cAMP binding; cAMP-dependent protein kinase regulator activity; nucleotide binding