Recombinant Human Proteasome (prosome, macropain) Subunit, Beta Type, 7, His-tagged

• Cat.No.: PSMB7-1933H
• Product Overview: Recombinant humanPSMB7 protein,fused to His-tag at N-terminus, was expressed in E. coli and purified by usingconventional chromatography.

Specification

• Species: Human
• Tag: His
• Description: PSMB7, also known asproteasome subunit beta type-7, is a multicatalytic proteinasecomplex with a highly ordered ring-shaped 20S core structure. The corestructure is composed of 4 rings of 28 non-identical subunits; 2 rings arecomposed of 7 alpha subunits and 2 rings are composed of 7 beta subunits.Proteasomes are distributed throughout eukaryotic cells at a highconcentration and cleave peptides in an ATP/ubiquitin-dependent process in anon-lysosomal pathway. An essential function of a modified proteasome, theimmunoproteasome, is the processing of class I MHC peptides. This geneencodes a member of the proteasome B-type family, also known as the T1Bfamily that is a 20S core beta subunit in the proteasome.
• Form: Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT.
• Molecular Weight: 27.6 kDa (255aa)confirmed by MALDI-TOF
• Purity: > 80%by SDS-PAGE
• Concentration: 0.25 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MTTIAGVVYK DGIVLGADTR ATEGMVVADK NCSKIHFISP NIYCCGAGTAADTDMTTQLI SSNLELHSLS TGRLPRVVTA NRMLKQMLFR YQGYIGAALV LGGVDVTGPH LYSIYPHGSTDKLPYVTMGS GSLAAMAVFE DKFRPDMEEE EAKNLVSEAI AAGIFNDLGS GSNIDLCVIS KNKLDFLRPYTVPNKKGTRL GRYRCEKGTT AVLTEKITPL EIEVLEETVQ TMDTS
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoidrepeated freezing and thawing cycles.
• Pathways: APC/C-mediated degradation of cell cycleproteins; APC/C:Cdc20 mediated degradation of Securin; APC/C:Cdc20 mediateddegradation of mitotic proteins; Adaptive Immune System; Antigenprocessing-Cross presentation; Apoptosis; Assembly of the pre-replicativecomplex; Cell Cycle Checkpoints; Cell Cycle; DNA Replication; DNA ReplicationPre-Initiation; ER-Phagosome pathway; G1/S DNA Damage Checkpoints; G1/STransition

Gene Information

• Gene Name: PSMB7 proteasome (prosome,macropain) subunit, beta type, 7 [ Homo sapiens ]
• Official Symbol: PSMB7
• Synonyms: PSMB7;proteasome (prosome, macropain) subunit, beta type, 7; Z;proteasome subunit beta type-7; macropain chain Z; proteasome subunit Z;proteasome subunit alpha; proteasome subunit beta 7; proteasome catalyticsubunit 2; multicatalytic endopeptidase complex chain Z; EC 3.4.25.1
• Gene ID: 5695
• mRNA Refseq: NM_002799
• Protein Refseq: NP_002790
• MIM: 604030
• UniProt ID: Q6IBT1
• Chromosome Location: 9q34.11-q34.12
• Function: peptidaseactivity ; protein binding ; threonine-type endopeptidase activity