Recombinant Human Proteasome (prosome, macropain) Inhibitor Subunit 1 (PI31), His-tagged

• Cat.No.: PSMF1-1567H
• Product Overview: Recombinant human PSMF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PSMF1 is a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1mM PMSF.
• Molecular Weight: 31.9 kDa (291aa), confirmed by MALDI-TOF.
• Purity: >85% by SDS-PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MAGLEVLFAS AAPAITCRQD ALVCFLHWEV VTHGYCGLGV GDQPGPNDKK SELLPAGWNN NKDLYVLRYEYKDGSRKLLV KAITVESSMI LNVLEYGSQQ VADLTLNLDD YIDAEHLGDF HRTYKNSEEL RSRIVSGIIT PIHEQWEKAN VSSPHREFPPATAREVDPLR IPPHHPHTSR QPPWCDPLGP FVVGGEDLDP FGPRRGGMIV DPLRSGFPRA LIDPSSGLPN RLPPGAVPPG ARFDPFGPIGTSPPGPNPDH LPPPGYDDMY L
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: APC/C-mediated degradation of cell cycle proteins; APC/C:Cdc20 mediated degradation of Securin; APC/C:Cdc20 mediated degradation of mitotic proteins; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins; Adaptive Immunity Signaling; Antigen processing: Ubiquitination& Proteasome degradation; Apoptosis; Assembly of the pre-replicative complex; Autodegradation of Cdh1 by Cdh1:APC/C; Autodegradation of the E3 ubiquitin ligase COP1; CDK-mediated phosphorylation and removal of Cdc6; CDT1 association with the CDC6:ORC:origin complex; Cell Cycle Checkpoints; Cell Cycle

Gene Information

• Gene Name: PSMF1 proteasome (prosome, macropain) inhibitor subunit 1 (PI31) [ Homo sapiens ]
• Official Symbol: PSMF1
• Synonyms: PSMF1; proteasome (prosome, macropain) inhibitor subunit 1 (PI31); PI31; proteasome inhibitor PI31 subunit; hPI31; OTTHUMP00000029952; OTTHUMP00000029955; proteasome inhibitor hP131 subunit
• Gene ID: 9491
• mRNA Refseq: NM_006814
• Protein Refseq: NP_006805
• UniProt ID: Q92530
• Chromosome Location: 20p13
• Function: endopeptidase inhibitor activity; protein binding