Recombinant Human Proteasome Alpha 7 Subunit, His-tagged

• Cat.No.: PSMA7-122H
• Product Overview: Recombinant PSMA7 protein was expressed in E.coli and purified by using conventional chromatography techniques, 30.0 kDa (268 aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Proteasome alpha 7, PSMA7, is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. PSMA7, belonged to the peptidase T1A family, is a 20S core alpha subunit of proteasome. This protein was found to interact specifically with two subdomains of HIF-1alpha and inhibited the transactivation function of HIF-1alpha under both normoxic and hypoxia-mimicking conditions.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSHMSYDRAITVF SPDGHLFQVE YAQEAVKKGS TAVGVRGRDI VVLGVEKKSV AKLQDERTVR KICALDDNVC MAFAGLTADA RIVINRARVE CQSHRLTVED PVTVEYITRY IASLKQRYTQ SNGRRPFGIS ALIVGFDFDG TPRLYQTDPS GTYHAWKANA IGRGAKSVRE FLEKNYTDEA IETDDLTIKL VIKALLEVVQ SGGKNIELAV MRRDQSLKIL NPEEIEKYVA EIEKEKEENE KKKQKKAS
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 M NaCl
• Purity: > 90% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PSMA7 proteasome (prosome, macropain) subunit, alpha type, 7 [ Homo sapiens ]
• Synonyms: C6; EC 3.4.25.1; HSPC; MGC3755; OTTHUMP00000031449; RC6-1; XAPC7; Proteasome subunit RC6-1; Proteasome subunit XAPC7; proteasome (prosome, macropain) subunit, alpha type, 7; proteasome alpha 7 subunit; proteasome subunit alpha 4; PSMA7; 5` partial
• Gene ID: 5688
• mRNA Refseq: NM_002792
• Protein Refseq: NP_002783
• MIM: 606607
• UniProt ID: O14818
• Chromosome Location: 20q13.33
• Pathway: Proteasome; Apoptosis; Cell Cycle Checkpoints; Cell Cycle, Mitotic; DNA Replication; HIV Infection; Metabolism of amino acids; Signaling by Wnt
• Function: identical protein binding; peptidase activity ;threonine-type endopeptidase activity