Recombinant Human Profilin 2, His-tagged

• Cat.No.: PFN2-2361H
• Product Overview: Recombinant human PFN2 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. 17.2 kDa (160aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PFN2 is a ubiquitous actin monomer-binding protein belonging to the profilin family. This protein is ubiquitously expressed with highest expression in kidney, brain and skeletal muscle. Like other members of the profilin family, PFN2 functions as an actin monomer-binding protein that influences the structure of the cytoskeleton by regulating actin polymerization in response to extracellular signals. At high concentrations, it prevents the polymerization of actin whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT.
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAGWQSYVDN LMCDGCCQEA AIVGYCDAKY V WA ATAGGVF QSITPIEIDM IVGKDREGFF TNGLALGAKK CSVIRDSLYV DGD CTMDIRT KSQGGEPTYN VAVGRAGRVL VFVMGKEGVH GGGLNKKAYS MA KYL RDSGF.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PFN2 profilin 2 [ Homo sapiens ]
• Synonyms: profilin 2; PFL; D3S1319E; PFN2; profilin-2; profilin II; Profilin II
• Gene ID: 5217
• mRNA Refseq: NM_002628
• Protein Refseq: NP_002619
• MIM: 176590
• UniProt ID: P35080
• Chromosome Location: 3q25.1-q25.2
• Pathway: Regulation of actin cytoskeleton; Axon guidance
• Function: phosphatidylinositol-4,5-bisphosphate binding; actin binding; protein binding