Recombinant Human PRMP1, His-tagged

• Cat.No.: PRMT1-59H
• Product Overview: Recombinant Human PRMT1 fused with His-MBP tag at N-terminus produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 750 amino acids and having a molecular mass of 84 kDa. The PRMT1 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: PRMT1-59H
• Description: PRMT1 Methylates (mono & asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in a glycine and arginine-rich domain (may methylate HNRNPA1 and histones). Methylates SUPT5H. The PRMT1 protein functions as a histone methyltransferase specific for H4. PRMT1 is an essential factor in oncogenesis and is a potential novel therapeutic target in cancer. PRMT1-mediated methylation serves as a positive modulator of IR/IRS-1/PI3K pathway and glucose uptake in skeletal muscle cells. PRMT1 arginine-methylates MRE11 therefore it regulates the activity of MRE11-RAD50-NBS1 complex during the intra-S-phase DNA damage checkpoint response. PRMT1 plays a post-translationally part in regulating the transcriptional activity. PRMT1 is found predominantly in the cytoplasma though a fraction of PRMT1 is located in the nucleus.
• Source: Escherichia Coli.
• Amino Acid Sequence: HHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEKPNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGI LCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLYAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYV HALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR.
• Physical Appearance: Sterile Filtered colorless solution.
• Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The PRMT1 solution (1 mg/ml) cotains 40 mM Tris pH 8.0, 100 mM NaCl, 4 mM MgCl₂, 2 mM DTT & 40% glycerol.
• Unit Definition: One unit will transfer 1pmol of methyl group to synthetic peptide of histone H4 for 10 minutes at 37°C.
• Specific Activity: 10,000 Units/ml.
• Stability: PRMT1 although stable 14°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Protein length: 1-353 a.a.

Gene Information

• Gene Name: PRMT1 protein arginine methyltransferase 1 [ Homo sapiens ]
• Synonyms: PRMT1; protein arginine methyltransferase 1; ANM1; HCP1; IR1B4; HRMT1L2; HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2; HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae); Protein arginine N-methyltransferase 1; Interferon receptor 1-bound protein 4; HMT2; EC 2.1.1
• Gene ID: 3276
• mRNA Refseq: NM_001536
• Protein Refseq: NP_001527
• MIM: 602950
• UniProt ID: Q99873
• Chromosome Location: 19q13.3
• Function: N-methyltransferase activity; protein binding; protein methyltransferase activity; transferase activity