Recombinant Human PRKAR1B, His-tagged

• Cat.No.: PRKAR1B-7015H
• Product Overview: Recombinant full-length human PRKAR1B was expressed in Sf9 insect cells using a C-terminal His tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: Full length
• Description: PRKAR1B is the type I-beta regulatory subunit of cyclic AMP-dependent protein kinase A (PKA) which is an essential enzyme in the cAMP signaling pathway. PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP. PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. PKA undergoes a dramatic conformational change upon complex formation with the catalytic subunit. PRKAR1B subunits can dimerize through an N-terminal motif and this dimerization is necessary for binding to PKA anchoring proteins (AKAPs) and targeting of PKA to its site of action.
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Molecular Mass: ~52 kDa
• Purity: >90%
• Applications: Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.2 μg/μl

Gene Information

• Gene Name: PRKAR1B protein kinase, cAMP-dependent, regulatory, type I, beta [ Homo sapiens ]
• Official Symbol: PRKAR1B
• Synonyms: PRKAR1B; protein kinase, cAMP-dependent, regulatory, type I, beta; cAMP-dependent protein kinase type I-beta regulatory subunit; PRKAR1
• Gene ID: 5575
• mRNA Refseq: NM_001164758
• Protein Refseq: NP_001158230
• MIM: 176911
• UniProt ID: P31321
• Chromosome Location: 7pter-p22
• Pathway: Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Aquaporin-mediated transport, organism-specific biosystem; Ca-dependent events, organism-specific biosystem; CaM pathway, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Calmodulin induced events, organism-specific biosystem
• Function: cAMP binding; cAMP-dependent protein kinase regulator activity; nucleotide binding