Recombinant Human PRKAA2, GST-tagged

• Cat.No.: PRKAA2-73H
• Product Overview: Recombinant full-length human AMPK (combination of A2/B2/G3 subunits) was expressed by baculovirus in Sf9 insect cells using the N-terminal GST and C-terminal His tags.

Specification

• Description: AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for the treatment of diabetes. AMPK is viewed as a fuel sensor for glucose and lipid metabolism by modulating the activity of the autonomous nervous system in vivo. Short-term overexpression of a constitutively active form of AMPK in the liver leads to mild hypoglycemia and fatty liver due to increased fatty acid utilization.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: His&GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~92kDa (A2), ~62kDa (B2), and ~108kDa (G3)
• Purity: >80% by densitometry.
• Applications: Kinase Assay, Western Blot
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: PRKAA2 protein kinase, AMP-activated, alpha 2 catalytic subunit [ Homo sapiens ]
• Official Symbol: PRKAA2
• Synonyms: PRKAA2; protein kinase, AMP-activated, alpha 2 catalytic subunit; PRKAA; 5-AMP-activated protein kinase catalytic subunit alpha-2; AMPK; AMPKa2; AMPK-alpha-2 chain; AMPK subunit alpha-2; 5-AMP-activated protein kinase, catalytic alpha-2 chain; AMPK2
• Gene ID: 5563
• mRNA Refseq: NM_006252
• Protein Refseq: NP_006243
• MIM: 600497
• UniProt ID: P54646
• Chromosome Location: 1p31
• Pathway: AMPK inhibits chREBP transcriptional activation activity, organism-specific biosystem; AMPK signaling, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Energy Metabolism, organism-specific biosystem; Energy dependent regulation of mTOR by LKB1-AMPK, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem
• Function: AMP-activated protein kinase activity; AMP-activated protein kinase activity; ATP binding; chromatin binding; histone serine kinase activity; metal ion binding; nucleotide binding; protein binding; protein binding, bridging; protein kinase activity; protein serine/threonine kinase activity