Active Recombinant Human AMPK (A2/B1/G3), GST-His-tagged

• Cat.No.: PRKAA2-79H
• Product Overview: Recombinant full-length human AMPK (combination of A2/B1/G3 subunits) was expressed by baculovirus in Sf9 insect cells using the N-terminal GST and C-terminal His tags.

Specification

• Description: AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory beta and gamma subunits. Binding of AMP to the gamma subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKKbeta.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: His&GST
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: The specific activity of AMPK was determined to be 360 nmol/min/mg
• Molecular Mass: ~92 kDa (A2), ~65 kDa (B1), and ~108 kDa (G3)
• Purity: >90% by densitometry.
• Applications: Kinase Assay, Western Blot
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: PRKAA2 protein kinase, AMP-activated, alpha 2 catalytic subunit [ Homo sapiens ]
• Official Symbol: PRKAA2
• Synonyms: PRKAA2; protein kinase, AMP-activated, alpha 2 catalytic subunit; PRKAA; 5-AMP-activated protein kinase catalytic subunit alpha-2; AMPK; AMPKa2; AMPK-alpha-2 chain; AMPK subunit alpha-2; 5-AMP-activated protein kinase, catalytic alpha-2 chain; AMPK2
• Gene ID: 5563
• mRNA Refseq: NM_006252
• Protein Refseq: NP_006243
• MIM: 600497
• UniProt ID: P54646
• Chromosome Location: 1p31
• Pathway: AMPK inhibits chREBP transcriptional activation activity, organism-specific biosystem; AMPK signaling, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Energy Metabolism, organism-specific biosystem; Energy dependent regulation of mTOR by LKB1-AMPK, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem
• Function: AMP-activated protein kinase activity; AMP-activated protein kinase activity; ATP binding; chromatin binding; histone serine kinase activity; metal ion binding; nucleotide binding; protein binding; protein binding, bridging; protein kinase activity; protein serine/threonine kinase activity