Recombinant Human PRKAA1 protein, His & T7-tagged

• Cat.No.: PRKAA1-775H
• Product Overview: Recombinant Human PRKAA1 aa. (Gly198~Lys448 (Accession # Q13131)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.2kDa
• Protein length: Gly198~Lys448
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.8.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PRKAA1 protein kinase AMP-activated catalytic subunit alpha 1 [ Homo sapiens (human) ]
• Official Symbol: PRKAA1
• Synonyms: AMPK; AMPKa1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; ACACA kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK subunit alpha-1; HMGCR kinase; acetyl-CoA carboxylase kinase; hydroxymethylglutaryl-CoA reductase kinase; protein kinase, AMP-activated, alpha 1 catalytic subunit; tau-protein kinase PRKAA1; 5'-AMP-activated protein kinase catalytic subunit alpha-1
• Gene ID: 5562
• mRNA Refseq: NM_006251.5
• Protein Refseq: NP_006242.5
• UniProt ID: Q13131