Recombinant Human PRG4 protein, His-tagged

• Cat.No.: PRG4-361H
• Product Overview: Recombinant Human PRG4(Val1151~Gly1241) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1151-1241 a.a.
• Description: The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants.
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Molecular Mass: 11.6kDa
• AA Sequence: MGHHHHHHSG S-VDGLTTLRNG TLVAFRGHYF WMLSPFSPPS PARRITEVWG IPSPIDTVFT RCNCEGKTFF FKDSQYWRFT NDIKDAGYPK PIFKGFGGLT G
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Applications: SDS-PAGE; WB; ELISA; IP.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PRG4 proteoglycan 4 [ Homo sapiens ]
• Official Symbol: PRG4
• Synonyms: PRG4; proteoglycan 4; CACP, proteoglycan 4, (megakaryocyte stimulating factor, articular superficial zone protein, camptodactyly, arthropathy, coxa vara, pericarditis syndrome); articular superficial zone protein; bG174L6.2; bG174L6.2 (MSF: megakaryocyte stimulating factor ); camptodactyly; arthropathy; coxa vara; pericarditis syndrome; FLJ32635; HAPO; Jacobs camptodactyly arthropathy pericarditis syndrome; JCAP; lubricin; megakaryocyte stimulating factor; MSF; SZP; superficial zone proteoglycan; megakaryocyte-stimulating factor; CACP
• Gene ID: 10216
• mRNA Refseq: NM_001127708
• Protein Refseq: NP_001121180
• MIM: 604283
• UniProt ID: Q92954
• Chromosome Location: 1q25-q31
• Function: polysaccharide binding; scavenger receptor activity