Recombinant Human PPP3R1 protein(Gly2-Val170), His-tagged

• Cat.No.: PPP3R1-7366H
• Product Overview: Recombinant Human PPP3R1 (P63098) (Gly2-Val170) was expressed in Insect Cells, fused with a polyhistide tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Protein length: Gly2-Val170
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol, 2mM DTT. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Using the Octet RED System, the affinity constant (Kd) of human PPP3R1-His bound to Human PPIA-His was 6 nM.
• Molecular Mass: The recombinant human PPP3R1 consists of 187 amino acids and has a calculated molecular mass of 21.4 kDa. The recombinant protein migrates as an approximately 20 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 87 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PPP3R1 protein phosphatase 3, regulatory subunit B, alpha [ Homo sapiens ]
• Official Symbol: PPP3R1
• Synonyms: PPP3R1; protein phosphatase 3, regulatory subunit B, alpha; protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform (calcineurin B, type I) , protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) , protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform; calcineurin subunit B type 1; calcineurin B; type I (19kDa); CALNB1; CNB; CNB1; protein phosphatase 2B regulatory subunit B alpha; calcineurin B, type I (19kDa); protein phosphatase 2B regulatory subunit 1; protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform; protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform (calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I)
• Gene ID: 5534
• mRNA Refseq: NM_000945
• Protein Refseq: NP_000936
• MIM: 601302
• UniProt ID: P63098