Recombinant Human PPP3R1, His-tagged

• Cat.No.: PPP3R1-3471H
• Product Overview: Recombinant human PPP3R1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: PPP3R1-3471H
• Description: PPP3R1, also known as Calcineurin subunit B type 1, is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway. Calcineurin is composed of two subunits; calcineurin A (CnA) and calcineurin B (CnB). Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
• Molecular Weight: 21.5 kDa (190aa), confirmed by MALDI-TOF
• Purity: > 90 % by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PPP3R1 protein phosphatase 3, regulatory subunit B, alpha [ Homo sapiens ]
• Synonyms: protein phosphatase 3, regulatory subunit B, alpha; CNB; CNB1; CALNB1; PPP3R1; calcineurin subunit B type 1; calcineurin B, type I (19kDa); protein phosphatase 2B regulatory subunit 1; protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform; protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform (calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I); OTTHUMP00000201960; OTTHUMP00000201961; Protein phosphatase 3 regulatory subunit B alpha isoform 1
• Gene ID: 5534
• mRNA Refseq: NM_000945
• Protein Refseq: NP_000936
• MIM: 601302
• UniProt ID: P63098
• Chromosome Location: 2p15
• Pathway: Amyotrophic lateral sclerosis (ALS); Alzheimer"s disease; Long-term potentiation; B cell receptor signaling pathway; T cell receptor signaling pathway; Natural killer cell mediated cytotoxicity; VEGF signaling pathway; Axon guidance; Wnt signaling pathway; Apoptosis; Oocyte meiosis; Calcium signaling pathway; MAPK signaling pathway; Apoptosis; Opioid Signalling
• Function: calcium ion binding; calcium-dependent protein serine/threonine phosphatase activity; calmodulin binding; protein binding