Recombinant Human PPP2CA Protein, octahistidine/streptactin-tagged

• Cat.No.: PPP2CA-077H
• Product Overview: Human recombinant PP2A catalytic subunit expressed in insect cells with an N-terminal octahistidine-tag followed by a streptactin-tag. The C-terminal leucine 309 was deleted.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His&Strep
• Description: This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
• Molecular Mass: 38.6 kDa
• Unit Definition: One unit of enzyme produces 1 μmol of p-nitrophenol per minute at 37 centigrade in 40 mM Tris-HCl H7.5, 34 mM MgCl2, 4mM EDTA, 2mM DTT, and 0.05 mg/mL BSA, containing 20 mM p-nitrophenyl phosphate.
• Stability: ≥ 1 year
• Storage: At -80 centigrade.
• Storage Buffer: 20 mM Tris, pH 7.5, with 100 mM sodium chloride, 5 mM MgCl2, 1 mM EDTA, and 25% glycerol

Gene Information

• Gene Name: PPP2CA protein phosphatase 2 catalytic subunit alpha [ Homo sapiens (human) ]
• Official Symbol: PPP2CA
• Synonyms: PPP2CA; protein phosphatase 2 catalytic subunit alpha; RP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; protein phosphatase 2, catalytic subunit, alpha isozyme; replication protein C; EC 3.1.3.16
• Gene ID: 5515
• mRNA Refseq: NM_002715
• Protein Refseq: NP_002706
• MIM: 176915
• UniProt ID: P67775