Recombinant Human PPME1, His-tagged

• Cat.No.: PPME1-3601H
• Product Overview: Recombinant human PPME1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (1-386aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PPME1, also known protein phosphatase methylesterase-1(PME1), catalyzes the demethylation and inactivation of protein phosphatase (PP2A), which is a multimeric phosphoserine/ threonine protein phosphatase associated with growth inhibition and cell cycle arrest. It can demethylate PP2A catalytic subunit in vitro and okadaic acid treatment is capable of inhibiting this reaction. It is conserved from yeast to human and contains a motif found in lipases having a catalytic triad activated serine as their active site nucleophile.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl,1mM DTT
• Molecular Weight: 44.4 kDa (406aa), confirmed by MALDI-TOF
• Purity: > 95% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSALEKSMHL GRLPSRPPLP GSGGSQSGAK MRMGPGRKRD FSPVPWSQYF ESMEDVEVEN ETGKDTFRVY KSGSEGPVLL LLHGGGHSAL SWAVFTAAII SRVQCRIVAL DLRSHGETKV KNPEDLSAET MAKDVGNVVE AMYGDLPPPI MLIGHSMGGA IAVHTASSNL VPSLLGLCMI DVVEGTAMDA LNSMQNFLRG RPKTFKSLEN AIEWSVKSGQ IRNLESARVS MVGQVKQCEG ITSPEGSKSI VEGIIEEEEE DEEGSESISK RKKEDDMETK KDHPYTWRIE LAKTEKYWDG WFRGLSNLFL SCPIPKLLLL AGVDRLDKDL TIGQMQGKFQ MQVLPQCGHA VHEDAPDKVA EAVATFLIRH RFAEPIGGFQ CVFPGC
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PPME1 protein phosphatase methylesterase 1 [ Homo sapiens ]
• Official Symbol: PPME1
• Synonyms: protein phosphatase methylesterase 1; PME-1; FLJ22226; PPME1; protein phosphatase methylesterase-1; EC=3.1.1.-; PP2593, PRO0750; OTTHUMP00000237973; OTTHUMP00000237974
• Gene ID: 51400
• mRNA Refseq: NM_016147
• Protein Refseq: NP_057231
• MIM: 611117
• UniProt ID: Q9Y570
• Chromosome Location: 11q13.4
• Function: carboxylesterase activity; hydrolase activity; protein C-terminal methylesterase activity; protein phosphatase 2A binding; protein phosphatase binding; protein phosphatase inhibitor activity; protein phosphatase type 2A regulator activity