Recombinant Human PPM1G, His-tagged

• Cat.No.: PPM1G-46H
• Product Overview: Recombinant Human protein phosphatase 1G fused with His-tag at N-terminus produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 250 amino acids and having a molecular mass of 27 kDa. The PRMT1 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: PPM1G-46H
• Description: PPM1G is part of the PP2C family of Ser/Thr protein phosphatases which are known to be negative regulators of cell stress response pathways. PPM1G is accountable for the dephosphorylation of Pre-mRNA splicing factors, an important factor for the formation of functional spliceosome. PPM1G regulates cell cycle progression. PPM1G mediates histone dephosphorylation/exchange in response to DNA damage or checkpoint recovery in higher eukaryotes. The degradation of p21/WAF1 induced by PPM1G is mediated in a proteasome-dependent manner. Protein phosphatase 1G regulates assembly and function of the beta-catenin degradation complex.
• Source: Escherichia Coli.
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEGKEEPGSD SGTTAVVALI RGKQLIVANA GDSRCVVSEA GKALDMSYDH KPEDEVELAR IKNAGGKVTM DGRVNGGLNL SRAIGDHFYK RNKNLPPEEQ MISALPDIKV LTLTDDHEFM VIACDGIWNV MSSQEVVDFI QSKISQRDEN GELRLLSSIV EELLDQCLAP DTSGDGTGCD NMTCIIICFK PRNTAELQPE SGKRKLEEVL STEGAEENGN SDKKKKAKRD.
• Physical Appearance: Sterile Filtered colorless solution.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The PPM1G solution (1 mg/ml) contains 25 mM Tris pH-7.5, 1 mM DTT, 1 mM EDTA, 2 mM β-ME and 20% glycerol.
• Stability: PPM1G although stable 14°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: PPM1G protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform [ Homo sapiens ]
• Synonyms: protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform; PP2CG; PPP2CG; MGC1675; MGC2870; PP2CGAMMA; PPM1G; protein phosphatase 1G; PP2C, gamma; protein phosphatase 1C; protein phosphatase 2C gamma isoform; protein phosphatase magnesium-dependent 1 gamma; protein phosphatase 2, catalytic subunit, gamma isoform; PP2Cgamma; PP2C-gamma; OTTHUMP00000123426; PPM1C; EC 3.1.3.16; Protein phosphatase 1C; Protein phosphatase 2C isoform gamma; Protein phosphatase magnesium-dependent 1 gamma
• Gene ID: 5496
• mRNA Refseq: NM_002707
• Protein Refseq: NP_002698
• MIM: 605119
• UniProt ID: O15355
• Function: protein serine/threonine phosphatase activity; manganese ion binding; magnesium ion binding; hydrolase activity