Recombinant Human PPAP2B 293 Cell Lysate

Description :	Antigen standard for phosphatidic acid phosphatase type 2B (PPAP2B), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source :	HEK 293 cells
Species :	Human
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag :	Non

Gene Name	PPAP2B phosphatidic acid phosphatase type 2B [ Homo sapiens ]
Official Symbol	PPAP2B
Synonyms	LPP3; VCIP; Dri42; PAP2B
Gene ID	8613
mRNA Refseq	NM_003713.4
Protein Refseq	NP_003704.3
MIM	607125
UniProt ID	O14495
Chromosome Location	1p32.2
Pathway	Ether lipid metabolism, organism-specific biosystem;Ether lipid metabolism, conserved biosystem;Fat digestion and absorption, organism-specific biosystem;Fat digestion and absorption, conserved biosystem;Fc gamma R-mediated phagocytosis, organism-specific biosystem;Fc gamma R-mediated phagocytosis, conserved biosystem;Glycerolipid metabolism, organism-specific biosystem;
Function	integrin binding;lipid phosphatase activity;phosphatidate phosphatase activity;phosphoprotein phosphatase activity;protein binding;