Recombinant Human PPAP2A 293 Cell Lysate

• Cat.No.: PPAP2A-2992HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for phosphatidic acid phosphatase type 2A (PPAP2A), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PPAP2A phosphatidic acid phosphatase type 2A [ Homo sapiens ]
• Official Symbol: PPAP2A
• Synonyms: PPAP2A; phosphatidic acid phosphatase type 2A; lipid phosphate phosphohydrolase 1; LPP1; PAP 2a; PAP2a; PAP2-alpha; phosphatidic acid phosphatase 2a; lipid phosphate phosphohydrolase 1a; phosphatidate phosphohydrolase type 2a; type 2 phosphatidic acid phosphohydrolase; phosphatidic acid phosphohydrolase type 2a; type-2 phosphatidic acid phosphatase alpha; PAP2; LLP1a; PAP-2a; PAP2a2; PAPalpha1; PAP2alpha2
• Gene ID: 8611
• mRNA Refseq: NM_003711
• Protein Refseq: NP_003702
• MIM: 607124
• UniProt ID: O14494
• Chromosome Location: 5q11
• Pathway: Ether lipid metabolism, organism-specific biosystem; Ether lipid metabolism, conserved biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Fc-epsilon receptor I signaling in mast cells, organism-specific biosystem
• Function: hydrolase activity; phosphatidate phosphatase activity; phosphatidate phosphatase activity