Recombinant Human POLB Protein, His tagged

• Cat.No.: POLB-620H
• Product Overview: Human DNA polymerase β (beta), also known as Pol beta and POLB, GenBank Accession No. NM_002690, a.a. 2-335(end), with N-terminal His-tag, expressed in an E. coli cell expression system.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2–335 aa
• Description: The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Aqueous buffer solution
• Molecular Mass: 39 kDa
• Purity: ≥80%
• Usage: Based on ProFoldin DNA Polymerase kit. DNA polymerase is incubated with dNTP mix and DNA substrate in 50 μL reaction at RT for 1 h. Detection done by addition of 200 μL reagent U (ProFoldin) and 40 μL 1× fluorescence dye. Read fluorescence at λexc=485 nm and λem=535 nm.
• Notes: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
• Storage: At least 6 months at -80 centigrade.
• Storage Buffer: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, 0.04% Tween-20, and 20% glycerol.
• Shipping: At -80 centigrade.

Gene Information

• Gene Name: POLB DNA polymerase beta [ Homo sapiens (human) ]
• Official Symbol: POLB
• Synonyms: POLB; polymerase (DNA directed), beta; DNA polymerase beta; DNA pol beta; DNA polymerase beta subunit; MGC125976
• Gene ID: 5423
• mRNA Refseq: NM_002690
• Protein Refseq: NP_002681
• MIM: 174760
• UniProt ID: P06746

Reviews

09/07/2023

Efficacy: The enzyme is still active and I am currently using it in some DNA repair assays. It works very well for single nucleotide insertions in gapped DNA which is mostly what I have been using it for recently. Quality: Very happy with the quality and longevity. I think the enzyme is ~1yr old with frequent usage and it is still working fine. Overall Experience: Very happy. When we need more polB/hUNG, and potentially others, we will purchase from you again.