Recombinant Human POLR2A, His-tagged

• Cat.No.: POLR2A-29986TH
• Product Overview: Recombinant His tagged fragment containing the carboxy-terminal repeat domain of Human RNA polymerase II isolated from an E. coli strain that carries the coding sequence of human RNA pol II CTD under the control of a T7 promoter.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
• Conjugation: HIS
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the RNA polymerase beta chain family.

Gene Information

• Gene Name: POLR2A polymerase (RNA) II (DNA directed) polypeptide A, 220kDa [ Homo sapiens ]
• Official Symbol: POLR2A
• Synonyms: POLR2A; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; POLR2, polymerase (RNA) II (DNA directed) polypeptide A (220kD); DNA-directed RNA polymerase II subunit RPB1; POLRA; RPB1
• Gene ID: 5430
• mRNA Refseq: NM_000937
• Protein Refseq: NP_000928
• Uniprot ID: P24928
• Chromosome Location: 17p13.1
• Pathway: Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; DNA Repair, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem; Formation and Maturation of mRNA Transcript, organism-specific biosystem
• Function: DNA binding; DNA-directed RNA polymerase activity; RNA-directed RNA polymerase activity; metal ion binding; nucleotidyltransferase activity