Recombinant Human POLR1E 293 Cell Lysate

• Cat.No.: POLR1E-3038HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for polymerase (RNA) I polypeptide E, 53kDa (POLR1E) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: POLR1E polymerase (RNA) I polypeptide E, 53kDa [ Homo sapiens ]
• Official Symbol: POLR1E
• Synonyms: POLR1E; polymerase (RNA) I polypeptide E, 53kDa; polymerase (RNA) I associated factor 1 , PRAF1; DNA-directed RNA polymerase I subunit RPA49; FLJ13390; FLJ13970; PAF53; RNA polymerase I associated factor 53; RNA polymerase I subunit A49; RNA polymerase I-associated factor 1; RNA polymerase I-associated factor 53; polymerase (RNA) I associated factor 1; DNA-directed RNA polymerase I subunit E; PRAF1; FLJ43482; RP11-405L18.3
• Gene ID: 64425
• mRNA Refseq: NM_022490
• Protein Refseq: NP_071935
• UniProt ID: Q9GZS1
• Chromosome Location: 9p13.1
• Pathway: Eukaryotic Transcription Initiation, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem; Pyrimidine metabolism, organism-specific biosystem; Pyrimidine metabolism, conserved biosystem; RNA polymerase, organism-specific biosystem
• Function: DNA binding; DNA-directed RNA polymerase activity