Recombinant Human POLR1D 293 Cell Lysate

Description :	Antigen standard for polymerase (RNA) I polypeptide D, 16kDa (POLR1D), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source :	HEK 293 cells
Species :	Human
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag :	Non

Gene Name	POLR1D polymerase (RNA) I polypeptide D, 16kDa [ Homo sapiens ]
Official Symbol	POLR1D
Synonyms	POLR1D; polymerase (RNA) I polypeptide D, 16kDa; DNA-directed RNA polymerases I and III subunit RPAC2; MGC9850; RPA9; RPA16; RPAC2; RPO1 3; RNA polymerases I and III subunit AC2; DNA-directed RNA polymerase I subunit D; AC19; TCS2; RPC16; POLR1C; RPO1-3; FLJ20616;
Gene ID	51082
mRNA Refseq	NM_015972
Protein Refseq	NP_057056
MIM	613715
UniProt ID	Q9Y2S0
Chromosome Location	13q12.2
Pathway	Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem; Gene Expression, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem;
Function	DNA binding; DNA-directed RNA polymerase activity;

COA