Recombinant Human POLD1 protein, His & GST-tagged

• Cat.No.: POLD1-8025H
• Product Overview: Recombinant Human POLD1 aa. (Met41~Phe254 (Accession # P28340)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Met41~Phe254
• Description: This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 53.4kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 4.9.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: POLD1 DNA polymerase delta 1, catalytic subunit [ Homo sapiens (human) ]
• Official Symbol: POLD1
• Synonyms: CDC2; MDPL; POLD; CRCS10; CDC2 homolog; DNA polymerase subunit delta p125; polymerase (DNA directed), delta 1, catalytic subunit 125kDa; polymerase (DNA) delta 1, catalytic subunit; DNA polymerase delta catalytic subunit
• Gene ID: 5424
• mRNA Refseq: NM_001256849.1
• Protein Refseq: NP_001243778.1
• UniProt ID: P28340