Recombinant Human Podoplanin (Fragment)

• Cat.No.: PDPN-956H
• Product Overview: Recombinant Human podoplanin (Fragment) produced inE.colistarted with GLST and ends with GLST. MW=25 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2). Their association is dependent on sialic acid on O-glycans of Podoplanin.
• Purity: >95%by SDS-PAGE and visualised by silver stain.
• Endotoxin level: <0.1ng/μg ofPodoplanin.
• Stabilizer: None.
• Buffer: PBS
• Formulation: Lyophilized.
• Reconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4℃ for 1 week or -20℃ for future use.
• Stability: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20℃. Reconstituted Podoplanin should be stored in working aliquots at -20℃. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: PDPN podoplanin [ Homo sapiens ]
• Synonyms: PDPN; podoplanin; T1A; GP36; GP40; Gp38; OTS8; T1A-2; AGGRUS; HT1A-1; PA2.26; lung type-I cell membrane-associated glycoprotein; hT1alpha-1; hT1alpha-2; OTTHUMP00000009640; OTTHUMP00000044504; glycoprotein, 36-KD; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); aggrus; PA2.26; Glycoprotein 36; PA2.26 antigen; T1-alpha; Aggrus; T1-alpha; lung type-I cell membrane-associated glycoprotein
• Gene ID: 10630
• mRNA Refseq: NM_001006624
• Protein Refseq: NP_001006625
• MIM: 608863
• UniProt ID: Q86YL7
• Chromosome Location: 1p36.21
• Function: NOT amino acid transmembrane transporter activity; NOT folic acid transporter activity; NOT water channel activity