Active Recombinant Mouse Pdpn protein, His&hFc-tagged

• Cat.No.: Pdpn-832M
• Product Overview: Recombinant Mouse Pdpn protein(NP_034459.2)(Met1-Leu141), fused with His and hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met1-Leu141
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized mouse PDPN-Fch at 10 μg/ml (100 μl/well) can bind biotinylated human CLEC1B-His, The EC50 of biotinylated human CLEC1B-His is 0.04-0.08 μg/ml.
• Molecular Mass: The recombinant mouse PDPN/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 367 amino acids and has a predicted molecular mass of 40.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmPDPN/Fc monomer is approximately 60-65 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Pdpn podoplanin [ Mus musculus ]
• Official Symbol: Pdpn
• Synonyms: PDPN; podoplanin; aggrus; PA2.26 antigen; glycoprotein 38; transmembrane glycoprotein E11; T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha
• Gene ID: 14726
• mRNA Refseq: NM_010329
• Protein Refseq: NP_034459