Recombinant Human PLA2G3 protein, His-tagged

• Cat.No.: PLA2G3-119H
• Product Overview: Recombinant Human PLA2G3 protein (Gly20-Val268) was fused to His-tag at the N-terminus and expressed in E.coli.

Specification

• Description: This gene encodes a protein that belongs to the secreted phospholipase A2 family, whose members include the bee venom enzyme. The encoded enzyme functions in lipid metabolism and catalyzes the calcium-dependent hydrolysis of the sn-2 acyl bond of phospholipids to release arachidonic acid and lysophospholipids. This enzyme acts as a negative regulator of ciliogenesis, and may play a role in cancer development by stimulating tumor cell growth and angiogenesis. This gene is associated with oxidative stress, and polymorphisms in this gene are linked to risk for Alzheimer's disease.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder. 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: Predicted Molecular Mass: 31.4 kDa; Accurate Molecular Mass: 33 kDa.
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: >90% as determined by SDS-PAGE.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others.
• Warning: The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.
• Protein length: 20-268 a.a.

Gene Information

• Gene Name: PLA2G3
• Official Symbol: PLA2G3
• Synonyms: SPLA2III; sPLA2-III; GIII-SPLA2
• Gene ID: 50487
• mRNA Refseq: NM_015715.5
• Protein Refseq: NP_056530.2
• MIM: 611651
• UniProt ID: Q9NZ20