Recombinant Human PLA2G3 293 Cell Lysate

• Cat.No.: PLA2G3-3142HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for phospholipase A2, group III (PLA2G3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PLA2G3 phospholipase A2, group III [ Homo sapiens ]
• Official Symbol: PLA2G3
• Synonyms: PLA2G3; phospholipase A2, group III; group 3 secretory phospholipase A2; GIII SPLA2; sPLA2-III; GIII sPLA2; group III secreted phospholipase A2; group III secretory phospholipase A2; phosphatidylcholine 2-acylhydrolase 3; phosphatidylcholine 2-acylhydrolase GIII; SPLA2III; GIII-SPLA2
• Gene ID: 50487
• mRNA Refseq: NM_015715
• Protein Refseq: NP_056530
• MIM: 611651
• UniProt ID: Q9NZ20
• Chromosome Location: 22q12.2
• Pathway: Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Ether lipid metabolism, organism-specific biosystem; Ether lipid metabolism, conserved biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; Fc epsilon RI signaling pathway, organism-specific biosystem
• Function: calcium ion binding; calcium-dependent phospholipase A2 activity; hydrolase activity