Recombinant Human PLA1A, His-tagged

• Cat.No.: PLA1A-168H
• Product Overview: PLA1A, 26-456aa, Human, His tag, E.coli

Specification

• Description: PLA1A is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine in liposomes. Three transcript variants encoding different isoforms have been found for this gene. Recombinant human PLA1A protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 49.5 kDa (454aa)
• Protein length: 26-456aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSDAPPTPQ PKCADFQSAN LFEGTDLKVQ FLLFVPSNPS CGQLVEGSSD LQNSGFNATL GTKLIIHGFR VLGTKPSWID TFIRTLLRAT NANVIAVDWI YGSTGVYFSA VKNVIKLSLE ISLFLNKLLV LGVSESSIHI IGVSLGAHVG GMVGQLFGGQ LGQITGLDPA GPEYTRASVE ERLDAGDALF VEAIHTDTDN LGIRIPVGHV DYFVNGGQDQ PGCPTFFYAG YSYLICDHMR AVHLYISALE NSCPLMAFPC ASYKAFLAGR CLDCFNPFLL SCPRIGLVEQ GGVKIEPLPK EVKVYLLTTS SAPYCMHHSL VEFHLKELRN KDTNIEVTFL SSNITSSSKI TIPKQQRYGK GIIAHATPQC QINQVKFKFQ SSNRVWKKDR TTIIGKFCTA LLPVNDREKM VCLPEPVNLQ ASVTVSCDLK IACV
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PLA1A phospholipase A1 member A [ Homo sapiens ]
• Official Symbol: PLA1A
• Synonyms: PLA1A; phospholipase A1 member A; ps PLA1; phosphatidylserine-specific phospholipase A1alpha; PSPLA1; PS-PLA1
• Gene ID: 51365
• mRNA Refseq: NM_001206960
• Protein Refseq: NP_001193889
• MIM: 607460
• UniProt ID: Q53H76
• Chromosome Location: 3q13.13-q13.2
• Function: hydrolase activity; phosphatidylcholine 1-acylhydrolase activity