Recombinant Human Pim-1 Oncogene, GST-His

• Cat.No.: PIM1-726H
• Product Overview: Recombinant Human PIM1, Amino acids M1-K313, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells. MW = 65,423 Da.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-313 a.a.
• Description: Pim-1 is a proto-oncogene which encodes for the serine/threonine kinase of the same name. The pim-1 oncogene was first described in relation to murine T-cell lymphomas, as it was the locus most frequently activated by the Moloney murine leukemia virus.[4] Subsequently, the oncogene has been implicated in multiple human cancers, including prostate cancer, acute myeloid leukemia and other hematopoietic malignancies.
• Purification: One-step affinity purification using GSH-agarose.
• Product Identity: PIM1 was confirmed as human PIM1 by mass spectroscopy LC-ESIMS/ MS (Protagen AG, Germany).
• Storage Buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.
• Concentration: 0.185 μg/μl (Bradford method using BSA [Sigma, cat#A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 273 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: PIM1 pim-1 oncogene [ Homo sapiens ]
• Synonyms: PIM1; pim-1 oncogene; PIM; Proto-oncogene serine/threonine-protein kinase Pim-1; EC 2.7.11.1
• Gene ID: 5292
• mRNA Refseq: NM_002648
• Protein Refseq: NP_002639
• MIM: 164960
• UniProt ID: P11309
• Chromosome Location: 6p21
• Pathway: Acute myeloid leukemia; Jak-STAT signaling pathway
• Function: ATP binding; nucleotide binding; transferase activity; manganese ion binding; protein serine/threonine kinase activity; metal ion binding; transcription factor binding