Recombinant Human PIM1 protein, His & GST-tagged

• Cat.No.: PIM1-7906H
• Product Overview: Recombinant Human PIM1 aa. (Tyr129~Leu268 (Accession # P11309)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 47.8kDa.
• Protein length: Tyr129~Leu268
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.8.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PIM1 Pim-1 proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ]
• Official Symbol: PIM1
• Synonyms: PIM1; Pim-1 proto-oncogene, serine/threonine kinase; PIM; serine/threonine-protein kinase pim-1; Oncogene PIM1; pim-1 kinase 44 kDa isoform; pim-1 oncogene (proviral integration site 1); proto-oncogene serine/threonine-protein kinase pim-1
• Gene ID: 5292
• mRNA Refseq: NM_001243186.1
• Protein Refseq: NP_001230115.1
• UniProt ID: P11309