Recombinant Human PIK3R4 293 Cell Lysate

• Cat.No.: PIK3R4-3183HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for phosphoinositide-3-kinase, regulatory subunit 4 (PIK3R4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PIK3R4 phosphoinositide-3-kinase, regulatory subunit 4 [ Homo sapiens ]
• Official Symbol: PIK3R4
• Synonyms: PIK3R4; phosphoinositide-3-kinase, regulatory subunit 4; phosphoinositide 3-kinase regulatory subunit 4; p150; VPS15; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; phosphoinositide 3-kinase adaptor protein; phosphatidylinositol 3-kinase-associated p150; phosphoinositide-3-kinase, regulatory subunit 4, p150; MGC102700
• Gene ID: 30849
• mRNA Refseq: NM_014602
• Protein Refseq: NP_055417
• MIM: 602610
• UniProt ID: Q99570
• Chromosome Location: 3q22.1
• Pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; Focal Adhesion, organism-specific biosystem; IRS-mediated signalling, organism-specific biosystem; IRS-related events, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity