Recombinant Human PIK3R1, His-tagged

• Cat.No.: PIK3R1-1079H
• Product Overview: Recombinant full-length mouse p110alpha and human p85alpha were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.

Specification

• Description: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.?
• Source: Sf9 insect cells
• Species: Human
• Tag: His
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 2mM DTT, 25% glycerol.
• Molecular Mass: ~111 kDa (p110α), ~86 kDa (p85α)
• Protein length: Full Length
• Purity: >90%
• Applications: Kinase Assay; Western Blot
• Stability: 1 yr at -70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha) [ Homo sapiens (human) ]
• Official Symbol: PIK3R1
• Synonyms: PIK3R1; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); p85; GRB1; p85-ALPHA; phosphatidylinositol 3-kinase regulatory subunit alpha; OTTHUMP00000161918; OTTHUMP00000161919; OTTHUMP00000161920; OTTHUMP00000226152; OTTHUMP00000226159; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; ptdIns-3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
• Gene ID: 5295
• mRNA Refseq: NM_181523
• Protein Refseq: NP_852664
• MIM: 171833
• UniProt ID: P27986
• Chromosome Location: 5q13.1
• Pathway: AMPK signaling; Acute myeloid leukemia; B Cell Receptor Signaling Pathway
• Function: ErbB-3 class receptor binding; insulin receptor binding; insulin-like growth factor receptor binding