Recombinant Human PIK3C2A Protein, His-tagged

• Cat.No.: PIK3C2A-551H
• Product Overview: Recombinant Human PIK3C2A(Phe172~Asn403) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Phe172~Asn403
• Description: The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 29.7kDa
• Identity: Reconstitute in PBS or others
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: PIK3C2A phosphoinositide-3-kinase, class 2, alpha polypeptide [ Homo sapiens ]
• Official Symbol: PIK3C2A
• Synonyms: PIK3C2A; phosphoinositide-3-kinase, class 2, alpha polypeptide; phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha; PI3K C2alpha; PI3K-C2alpha; PI3K-C2-alpha; ptdIns-3-kinase C2 subunit alpha; phosphoinositide 3-kinase-C2-alpha; C2-containing phosphatidylinositol kinase; CPK; PI3-K-C2A; MGC142218; PI3-K-C2(ALPHA); DKFZp686L193
• Gene ID: 5286
• mRNA Refseq: NM_002645
• Protein Refseq: NP_002636
• MIM: 603601
• UniProt ID: O00443