Active Recombinant Human PIK3C2A, GST-tagged

• Cat.No.: PIK3C2A-1452H
• Product Overview: Recombinant human PIK3C2A (299-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 299 aa-end
• Description: PIK3C2A is belongs to the phosphoinositide 3-kinase (PI3K) family which play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. PIK3C2A can phosphorylate phosphatidylinositol and phosphatidylinositol-4-phosphate in vitro. PIK3C2A contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases that act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 22 nmol/min/mg
• Molecular Mass: ~155 kDa
• Purity: >70%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: PIK3C2A phosphoinositide-3-kinase, class 2, alpha polypeptide [ Homo sapiens ]
• Official Symbol: PIK3C2A
• Synonyms: PIK3C2A; phosphoinositide-3-kinase, class 2, alpha polypeptide; phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha; PI3K C2alpha; PI3K-C2alpha; PI3K-C2-alpha; ptdIns-3-kinase C2 subunit alpha; phosphoinositide 3-kinase-C2-alpha; C2-containing phosphatidylinositol kinase; CPK; PI3-K-C2A; MGC142218; PI3-K-C2(ALPHA); DKFZp686L193
• Gene ID: 5286
• mRNA Refseq: NM_002645
• Protein Refseq: NP_002636
• MIM: 603601
• UniProt ID: O00443
• Chromosome Location: 11p15.5-p14
• Pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; Clathrin derived vesicle budding, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem; Inositol phosphate metabolism, organism-specific biosystem; Inositol phosphate metabolism, conserved biosystem; Insulin Signaling, organism-specific biosystem
• Function: 1-phosphatidylinositol-3-kinase activity; 1-phosphatidylinositol-4-phosphate 3-kinase activity; ATP binding; binding; nucleotide binding; phosphatidylinositol 3-kinase activity; phosphatidylinositol binding; phosphotransferase activity, alcohol group as acceptor