Recombinant Human PIAS3 protein, His & GST-tagged

• Cat.No.: PIAS3-1245H
• Product Overview: Recombinant Human PIAS3 aa. (Val11~Leu280 (Accession # Q9Y6X2) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Val11~Leu280
• Description: This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 59.9kDa.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 9.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PIAS3 protein inhibitor of activated STAT 3 [ Homo sapiens (human) ]
• Official Symbol: PIAS3
• Synonyms: PIAS3; protein inhibitor of activated STAT 3; ZMIZ5; E3 SUMO-protein ligase PIAS3; protein inhibitor of activated STAT protein 3; zinc finger, MIZ-type containing 5
• Gene ID: 10401
• mRNA Refseq: NM_006099.3
• Protein Refseq: NP_006090.2
• UniProt ID: Q9Y6X2