Recombinant Human PIAS3 293 Cell Lysate

• Cat.No.: PIAS3-3202HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for protein inhibitor of activated STAT, 3 (PIAS3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PIAS3 protein inhibitor of activated STAT, 3 [ Homo sapiens ]
• Official Symbol: PIAS3
• Synonyms: PIAS3; protein inhibitor of activated STAT, 3; E3 SUMO-protein ligase PIAS3; FLJ14651; zinc finger; MIZ type containing 5; ZMIZ5; zinc finger, MIZ-type containing 5; protein inhibitor of activated STAT protein 3
• Gene ID: 10401
• mRNA Refseq: NM_006099
• Protein Refseq: NP_006090
• MIM: 605987
• UniProt ID: Q9Y6X2
• Chromosome Location: 1q21
• Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem; Hepatitis C, organism-specific biosystem; Hepatitis C, conserved biosystem; IL6-mediated signaling events, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem
• Function: SUMO ligase activity; enzyme binding; ligase activity; metal ion binding; nucleic acid binding; potassium channel regulator activity; protein C-terminus binding; protein N-terminus binding; protein binding; zinc ion binding