Recombinant Human PIAS2 293 Cell Lysate

• Cat.No.: PIAS2-3203HCL

Specification

• Description: Antigen standard for protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC212659, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: PIAS2 protein inhibitor of activated STAT, 2 [ Homo sapiens ]
• Official Symbol: PIAS2
• Synonyms: PIAS2; protein inhibitor of activated STAT, 2; E3 SUMO-protein ligase PIAS2; miz; PIASX ALPHA; PIASX BETA; zinc finger; MIZ type containing 4; ZMIZ4; DAB2-interacting protein; zinc finger, MIZ-type containing 4; msx-interacting zinc finger protein; protein inhibitor of activated STAT X; androgen receptor-interacting protein 3; DIP; MIZ; MIZ1; SIZ2; ARIP3; PIASX; PIASX-BETA; PIASX-ALPHA; MGC102682
• Gene ID: 9063
• mRNA Refseq: NM_004671
• Protein Refseq: NP_004662
• MIM: 603567
• UniProt ID: O75928
• Chromosome Location: 18q12.1-q12.3
• Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Hepatitis C, organism-specific biosystem; Hepatitis C, conserved biosystem; IL12 signaling mediated by STAT4, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem; Pathways in cancer, organism-specific biosystem
• Function: DNA binding; SUMO ligase activity; androgen receptor binding; ligase activity; metal ion binding; protein binding; transcription coactivator activity; ubiquitin protein ligase binding; zinc ion binding