Recombinant Human Phospholysine PhosphoHistidine Inorganic Pyrophosphate Phosphatase, His-tagged

• Cat.No.: LHPP-1319H
• Product Overview: Recombinant human LHPP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: LHPP is a member of the HAD-like hydrolase superfamily. It is a unique enzyme that hydrolyzes not only oxygen-phosphorus bonds in inorganic pyrophosphate but also nitrogen-phosphorus bonds in phospholysine, phosphohistidine and imidodiphosphate in vitro. LHPP is expressed in liver, kidney and moderately in brain.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol, 0.1 M NaCl.
• Molecular Weight: 33.5 kDa (307 aa) confirmed by MALDI-TOF
• Purity: > 90 % by SDS-PAGE
• Concentration: 1 mg/ml (determined by Bradford)
• Sequences of amino acids: MRGSHHHHHP WYASMTGGQQ MGRDLYDDDD KDRWGSHMAP WGKRLAGVRG VLLDISGVLY DSGAGGGTAI AGSVEAVARL KRSRLKVRFC TNESQKSRAE LVGQLQRLGF DISEQEVTAP APAACQILKE QGLRPYLLIH DGVRSEFDQI DTSNPNCVVI ADAGESFSYQ NMNNAFQVLM ELEKPVLISL GKGRYYKETS GLMLDVGPYM KALEYACGIK AEVVGKPSPE FFKSALQAIG VEAHQAVMIG DDIVGDVGGA QRCGMRALQV RTGKFRPSDE HHPEVKADGY VDNLAEAVDL LLQHADK
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Oxidative phosphorylation

Gene Information

• Gene Name: LHPP phospholysine phosphohistidine inorganic pyrophosphate phosphatase [ Homo sapiens ]
• Official Symbol: LHPP
• Synonyms: LHPP; phospholysine phosphohistidine inorganic pyrophosphate phosphatase; HDHD2B; FLJ44846; FLJ46044; MGC117251; MGC142189; MGC142191; phospholysine phosphohistidine inorganic pyrophosphate phosphatase; Hlhpp; OTTHUMP00000020691; OTTHUMP00000020692; OTTHUMP00000020693; NP_001161352.1; EC 3.6.1.1
• Gene ID: 64077
• mRNA Refseq: NM_001167880
• Protein Refseq: NP_001161352
• UniProt ID: Q9H008
• Chromosome Location: 10q26.2
• Function: hydrolase activity; inorganic diphosphatase activity; magnesium ion binding; phosphohistidine phosphatase activity; protein homodimerization activity