Recombinant Human Phosphoinositide-3-Kinase, Regulatory Subunit 1 (Alpha), His-tagged

• Cat.No.: PIK3R1-4864H
• Product Overview: Recombinant full-length human PIK3R1 (p85alpha) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Cat. No.: PIK3R1-4864H
• Description: The PIK3R1 comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. p85α modulates the interaction between PIK3R1 and platelet-derived growth factor receptor. Furthermore, estrogen receptor isoform ER-alpha binds in a ligand-dependent manner to the p85-alpha regulatory subunit of PIK3R1. Stimulation with estrogen increases ER-alpha-associated PIK3R1 activity, leading to the activation of protein kinase B/AKT and endothelial nitric oxide synthase (eNOS).
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 86 kDa
• Expression System: Sf9 insect cells using baculovirus
• Species: Human
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
• Purity: > 95 %
• Concentration: 0.2 ug/ul
• Sequences: Full-length
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: 3-phosphoinositide biosynthesis; Acute myeloid leukemia; Adaptive Immunity Signaling; B cell receptor signaling pathway; BCR signaling pathway; CD28 co-stimulation; CXCR3-mediated signaling events; Colorectal cancer; Downstream signaling of activated FGFR; EPO signaling pathway; Fc-epsilon receptor I signaling in mast cells; Glioma; IGF1 pathway; IRS-related events; Kit Receptor Signaling Pathway

Gene Information

• Gene Name: PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha) [ Homo sapiens ]
• Official Symbol: PIK3R1
• Synonyms: PIK3R1; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); p85; GRB1; p85-ALPHA; phosphatidylinositol 3-kinase regulatory subunit alpha; OTTHUMP00000161918; OTTHUMP00000161919; OTTHUMP00000161920; OTTHUMP00000226152; OTTHUMP00000226159; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; ptdIns-3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
• Gene ID: 5295
• mRNA Refseq: NM_181523
• Protein Refseq: NP_852664
• MIM: 171833
• UniProt ID: P27986
• Chromosome Location: 5q13.1
• Function: 1-phosphatidylinositol binding; ErbB-3 class receptor binding; insulin binding; insulin receptor binding; insulin receptor substrate binding; insulin-like growth factor receptor binding; phosphatidylinositol 3-kinase regulator activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein phosphatase binding