Recombinant Human PGBD1 293 Cell Lysate

Description :	Antigen standard for piggyBac transposable element derived 1 (PGBD1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source :	HEK 293 cells
Species :	Human
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag :	Non

Gene Name	PGBD1 piggyBac transposable element derived 1 [ Homo sapiens ]
Official Symbol	PGBD1
Synonyms	PGBD1; piggyBac transposable element derived 1; piggyBac transposable element-derived protein 1; dJ874C20.4; HUCEP 4; SCAND4; cerebral protein 4; cerebral protein-4; HUCEP-4;
Gene ID	84547
mRNA Refseq	NM_001184743
Protein Refseq	NP_001171672
UniProt ID	Q96JS3
Chromosome Location	6p22.1
Function	scavenger receptor activity; sequence-specific DNA binding transcription factor activity;