Recombinant Human PFKFB2, GST-tagged

• Cat.No.: PFKFB2-67H
• Product Overview: Recombinant full-length human PFKFB2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: PFKFB2 is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. PFKFB2 has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. PFKFB2 regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. PFKFB2 also functions as a homodimer. PFKFB2 play an important role in the regulation of muscle glycolysis during the first moments of exercise.
• Form: 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~84 kDa
• Applications: Western Blot
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: PFKFB2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 [ Homo sapiens ]
• Official Symbol: PFKFB2
• Synonyms: PFK-2/FBPase-2
• Gene ID: 5208
• mRNA Refseq: NM_006212.2
• Protein Refseq: NP_006203.2
• MIM: 171835
• UniProt ID: O60825
• Chromosome Location: 1q31
• Pathway: Fructose and mannose metabolism, organism-specific biosystem;Fructose and mannose metabolism, conserved biosystem;Glucose metabolism, organism-specific biosystem;Glycolysis, organism-specific biosystem;Metabolism, organism-specific biosystem;Metabolism of carbohydrates, organism-specific biosystem
• Function: 6-phosphofructo-2-kinase activity;ATP binding;fructose-2,6-bisphosphate 2-phosphatase activity;protein binding;protein kinase binding