Recombinant Human PFKFB1, GST-tagged

• Cat.No.: PFKFB1-214H
• Product Overview: Recombinant full-length human PFKFB1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: PFKFB1 is a member of the family of bifunctional 6-phosphofructo-2-kinase: fructose-2, 6-biphosphatase enzymes which forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Regulation of fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis.
• Form: Recombinant protein stored in 50mM Tis-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~80 kDa
• Purity: >95% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: PFKFB1?6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 [Homo sapiens?(human) ]
• Official Symbol: PFKFB1
• Synonyms: PFKFB1; F6PK; HL2K; PFRX; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1; PFK/FBPase 1; 6PF-2-K/Fru-2,6-P2ase 1; 6PF-2-K/Fru-2,6-P2ASE liver isozyme; fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase; EC 2.7.1.105; EC 3.1.3.46
• Gene ID: 5207
• mRNA Refseq: NM_001271804
• Protein Refseq: ?NP_001258733
• MIM: 311790
• UniProt ID: P16118
• Chromosome Location: Xp11.21
• Pathway: Fructose and mannose metabolism; Gluconeogenesis; Glucose metabolism
• Function: 6-phosphofructo-2-kinase activity; ATP binding; fructose-2,6-bisphosphate 2-phosphatase activity