Recombinant Human PECR 293 Cell Lysate

• Cat.No.: PECR-3309HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for peroxisomal trans-2-enoyl-CoA reductase (PECR) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PECR peroxisomal trans-2-enoyl-CoA reductase [ Homo sapiens ]
• Official Symbol: PECR
• Synonyms: PECR; peroxisomal trans-2-enoyl-CoA reductase; HSA250303; SDR29C1; short chain dehydrogenase/reductase family 29C; member 1; TERP; DCR-RP; pVI-ARL; 2,4-dienoyl-CoA reductase-related protein; putative short chain alcohol dehydrogenase; short chain dehydrogenase/reductase family 29C, member 1; DCRRP; PVIARL; HPDHASE
• Gene ID: 55825
• mRNA Refseq: NM_018441
• Protein Refseq: NP_060911
• MIM: 605843
• UniProt ID: Q9BY49
• Chromosome Location: 2q35
• Pathway: Biosynthesis of unsaturated fatty acids, organism-specific biosystem; Biosynthesis of unsaturated fatty acids, conserved biosystem; Fatty Acid Biosynthesis, organism-specific biosystem; Mitochondrial LC-Fatty Acid Beta-Oxidation, organism-specific biosystem; Peroxisome, organism-specific biosystem; Peroxisome, conserved biosystem
• Function: nucleotide binding; oxidoreductase activity; trans-2-enoyl-CoA reductase (NADPH) activity