Recombinant Human PDK3 293 Cell Lysate

• Cat.No.: PDK3-3328HCL

Specification

• Description: Antigen standard for pyruvate dehydrogenase kinase, isozyme 3 (PDK3), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: PDK3 pyruvate dehydrogenase kinase, isozyme 3 [ Homo sapiens ]
• Official Symbol: PDK3
• Synonyms: PDK3; pyruvate dehydrogenase kinase, isozyme 3; pyruvate dehydrogenase kinase, isoenzyme 3; pyruvate dehydrogenase, lipoamide, kinase isozyme 3, mitochondrial
• Gene ID: 5165
• mRNA Refseq: NM_001142386
• Protein Refseq: NP_001135858
• MIM: 602526
• UniProt ID: Q15120
• Chromosome Location: Xp22.12
• Pathway: Metabolism, organism-specific biosystem; Pyruvate metabolism, organism-specific biosystem; Pyruvate metabolism and Citric Acid (TCA) cycle, organism-specific biosystem; Regulation of pyruvate dehydrogenase (PDH) complex, organism-specific biosystem; TCA Cycle, organism-specific biosystem; The citric acid (TCA) cycle and respiratory electron transport, organism-specific biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity; transferase activity; two-component sensor activity