Recombinant Human PDE6D, His-tagged

• Cat.No.: PDE6D-2407H
• Product Overview: Recombinant human PDE6D, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques, 18.4 kDa (158aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Human PDE6D was originally identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. Catalytically active PDE6 is a heterodimer (αβ) that is regulated by two inhibitory γ subunits. As PDE6D does not modify the catalytic properties of PDEαβ, its function is still unresolved.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT, 100mM NaCl.
• Purity: > 90% by SDS – PAGE.
• Concentration: 0.5mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MSAKDERARE ILRGFKLNWM NLRDAETGKI LWQGTEDLSV PGVEHEARVP KKILKCKAVS RELNFSSTEQ MEKFRLEQKV YFKGQCLEEW FFEFGFVIPN STNTWQSLIE AAPESQMMPA SVLTGNVIIE TKFFDDDLLV STSRVRLFYV GSHHHHHH.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PDE6D phosphodiesterase 6D, cGMP-specific, rod, delta [ Homo sapiens ]
• Synonyms: phosphodiesterase 6D, cGMP-specific, rod, delta; PDED; PDE6D; retinal rod rhodopsin-sensitive cGMP 3",5"-cyclic phosphodiesterase subunit delta; protein p17; GMP-PDE delta; phosphodiesterase 6D; Protein p17
• Gene ID: 5147
• mRNA Refseq: NM_002601
• Protein Refseq: NP_002592
• MIM: 602676
• UniProt ID: O43924
• Chromosome Location: 2q35-q36
• Pathway: Purine metabolism
• Function: 3",5"-cyclic-nucleotide phosphodiesterase activity; protein binding